浅析“3S”技术在森林资源调查规划中的应用分析

随着我国现代科学技术的不断进步,各种高新信息技术被广泛应用于林业工作,其中最明显的表现就是"3S"技术在地区森林资源调查规划中发挥重要技术支持作用。本文将以河南省南阳市为例,首先阐述了河南省南阳市森林资源概况及"3S"技术的技术原理,以南阳市林业调查实践操作为基础详细分析"3S"技术在现代森林资源调查规划(如:森林资源、森林病虫害、森林火灾及资源清查和绘制专题图等)的技术特征与优势,旨在进一步推动"3S"技术能够更加广泛、成熟的服务于我国森林资源调查规划活动,提高我国森林资源调查规划活动的准确性、高效性。     

哀牢山保护区景东片区中山湿性常绿阔叶林特点研究

哀牢山位于云南省中部,保护区分布在哀牢山的中上部,其中景东片区面积为11 812hm2,位于哀牢山西坡北段。此次我们在哀牢山保护区主要保护对象之一的中山湿性常绿阔叶林中设置了样地,开展详细调查,并对调查数据进行了计算、统计、分析。通过各个样本乔木层、灌木层、草本层的H’值比较,发现乔木层﹥灌木层﹥草本层,表明乔木层的信息量大于灌木层,而草本层是该结构中相对较低的。     

Identification of a mitochondrial‐targeting secretory protein from Nocardia seriolae which induces apoptosis in fathead minnow cells

Nocardia seriolae is the main pathogen responsible for fish nocardiosis. A mitochondrial‐targeting secretory protein (MTSP) 3141 with an N‐terminal transit peptide (TP) from N. seriolae was predicted by bioinformatic analysis based on the genomic sequence of the N. seriolae strain ZJ0503. However, the function of the MTSP3141 and its homologs remains totally unknown. In this study, mass spectrometry analysis of the extracellular products from N. seriolae proved that MTSP3141 was a secretory protein, subcellular localization research showed the MTSP3141‐GFP fusion protein co‐localized with mitochondria in fathead minnow (FHM) cells, the TP played an important role in mitochondria targeting, and only the TP located at N‐terminus but not C‐terminus can lead to mitochondria directing. Moreover, quantitative assays of mitochondrial membrane potential (ΔΨm) value, caspase‐3 activity and apoptosis‐related gene (Bcl‐2, Bax, Bad, Bid and p53) mRNA expression suggested that cell apoptosis was induced in FHM cells by the overexpression of both MTSP3141 and MTSP3141ΔTP (with the N‐terminal TP deleted) proteins. Taken together, the results of this study indicated that the MTSP3141 of N. seriolae was a secretory protein, might target mitochondria, induce apoptosis in host cells and function as a virulence factor.     

Changes in soil macropores: Superposition of the roles of organic nutrient amendments and the greenhouse pattern in vegetable plantations

Organic farming can potentially mitigate soil compaction, which commonly occurs in vegetable plantations, particularly in greenhouses when compared with open‐air systems. Although several studies have addressed the effect of planting patterns on soil pore characteristics, few studies have focused on changes in pore attributes under organic fertilization. We used adjacent fields during green manure planting as a control to compare the differences in soil physicochemical and macropore parameters between 14‐year‐old greenhouse plots and open‐air plots under organic fertilization. Pearson's correlation analysis was performed to analyse the drivers of soil macropore characteristics in vegetable plantations in Jiangsu Province, China. A significant increase in connectivity, fractal dimensions and total macroporosity and a decrease in bulk density after 14 years of organic farming, particularly in the surface layer in the greenhouse, were observed. However, the difference in pores in the plough pan layer was not significant between the treatments. The volume of small pores (50–500 μm) and medium pores (500–1,000 μm) increased significantly (p < 0.05) in both the greenhouse and open‐air systems, showing significantly positive relationships with soil organic matter (SOM) and total nitrogen (TN) content. Large pores (>1,000 μm) showed a clear decrease, especially in open‐air fields, possibly due to the disappearance of the original straw residues in the surface layer. Small and medium pores in the plough layer increased in greenhouse fields, whereas the opposite occurred in open‐air fields. Overall, compared with open‐air systems, long‐term organic greenhouse patterns had a significantly positive effect on soil pore attributes.     

Microtopographic structures facilitate plant recruitment across a saltmarsh tidal gradient

1. It is well known that establishment limitation is stronger than seed limitation in aquatic ecosystems. Therefore, it seems crucial during the recruitment recovery process to overcome this establishment limitation on bare or degraded patches.
2. Microtopographic structures have been shown to act as trap agents in saltmarshes. They can facilitate establishment, but this effect has not been quantitatively examined and assessed. This study examined the facilitative effect of microtopographic structures on plant recruitment in a dynamic saltmarsh system by conducting a series of field experiments along a saltmarsh tidal gradient.
3. A simple plant life‐cycle model was used to evaluate the dynamics of the plant recruitment process facilitated by microtopographic structure and the importance of each life stage. The influence of abiotic factors on each life stage was also assessed, to identify the determinants during the life history.
4. It was revealed that seed retention was the limiting factor in a bare saltmarsh area, rather than seed dispersal. Microtopographic structures can provide trap agents to facilitate seed anchorage. During seed retention, the stability of the microtopographic structure, especially its relative surface elevation difference, can be influenced by tidal events and the associated sediment process, which then affects seed retention efficiency.
5. Microtopographic structure has a strong environmental filtering effect that can revise the potential dispersed seed and emergence patterns, and the determinant of the final establishment pattern along a tidal gradient is the retention pattern resulting from the interactions between microtopographic structures and tidal events.
6. Furthermore, it can be designed into the early stage of recovery or restoration process so that it facilitates pioneer plant establishment. These early recruitment patches will speed up the recovery or succession process by (1) providing local seed sources, (2) retaining more seeds via the established vegetative structures, and (3) modifying microhabitat factors.

     

Effect of dietary inclusion of cottonseed meal on growth performance and physiological and immune responses in juvenile grass carp,Ctenopharyngodon idellus

An 8‐week feeding trial was conducted to evaluate the effect of replacement of dietary fishmeal with cottonseed meal (CSM) on the growth performance and health status of juvenile grass carp, Ctenopharyngodon idellus (6.67 g). Six isonitrogenous and isoenergetic diets were formulated with different CSM levels (0, 121, 241, 362, 482 and 603 g/kg diet). The first limiting amino acid was methionine, which decreased with an increase in dietary CSM from 361 to 538 g/kg. When the dietary CSM content was lower than 241 g/kg, the limiting amino acid was histidine. Thermal growth coefficient (TGC) and feed efficiency first increased and then decreased (p < 0.05), which was consistent with the change in the limiting amino acid. The feeding rate increased with a rise in the dietary CSM level (p < 0.05). The activity of digestive enzymes except trypsin decreased significantly (p < 0.05), serum aminotransferase activity and triglycerides increased, serum cholesterol decreased (p < 0.05). When dietary CSM was raised to 362 g/kg, intestinal villi and hepatopancreas tissue were injured. When the dietary CSM was 603 g/kg, alternative complement pathway haemolytic activity was inhibited (p < 0.05). The gossypol level in dorsal muscles from each treatment was below the detection limit. Based on the quadratic regression analysis of the TGC, the optimal dietary CSM level was 175.9 g/kg, and the maximum could be up to 351.8 g/kg and still result in the same TGC as that of control group.     

Response of N2O emission to straw retention under two contrasting paddy water conditions: a field experiment over rice–rice-fallow rotation

Paddy and Water Environment - Few studies are available on comprehensive impacts of straw retention and water regimes on nitrous oxide (N2O) emission from rice–rice-fallow rotation systems. A...     

禽肾炎病毒与鸡细小病毒双重PCR检测方法的建立

为了同时检测禽肾炎病毒(avian nephritis virus,ANV)和鸡细小病毒(chicken parvovirus,ChPV),根据GenBank中ANV的ORF基因和ChPV的NS1基因的保守序列,设计筛选了扩增片段大小分别为511 bp和242 bp的特异性引物,通过反应条件优化、特异性和敏感性试验,建立了一种双重PCR检测方法。该方法最佳引物比例为ANV上下引物各0.4μL(20 mol/L),ChPV上下引物各0.6μL(20 mol/L);最佳退火温度为53.8℃;灵敏度可达到55 fg(ANV)和58 fg(ChPV);对常见鸡病病原体进行检测,结果全为阴性。本研究建立的PCR方法具有特异、敏感、快速、稳定的优点,可用于同时鉴别诊断ANV和ChPV的混合感染。     

重庆市丘陵山区绿肥机械化轻简生产实践

针对高标准农田建设宜机化地块整理整治后的土壤培肥熟化问题，及近年来因过度施用化肥而导致的土壤酸化、板结等问题，重庆市通过绿肥种植试验示范，结合土壤环境保护和机械化生产技术，创新探索出了绿肥机械化轻简生产技术。其主要体现为2次机械化作业（抛撒和深松整地）完成传统生产方式中的6个作业环节（灭茬、旋耕、开沟、播种、施肥和盖种）。绿肥机械化轻简生产可减少化肥施用量，节约生产成本，保持和提高耕地质量，保护和改善生态环境，保证和提高农产品的产量及品质，促进农业的可持续发展。      

地源热泵稻谷干燥机板翅换热器的设计与试验

针对稻谷干燥机作业成本过高和干燥后的物料品质差的问题,将地源热泵技术应用于稻谷干燥机,设计了一套板翅式换热器加热空气,计算得出芯体结构与技术参数,以降低单位作业量成本,提高干燥后的物料品质。试验研究表明:当环境温度为环境温度16℃、空气相对湿度53.2%时,地源热泵稻谷干燥机与燃油稻谷干燥机的稻谷含水率降低速率无明显区别,稻谷干燥性能可达到同等效果;地源热泵稻谷干燥机干燥稻谷的单位作业量成本0.040 7元,而燃油稻谷干燥机干燥稻谷的单位作业量成本为0.190 5元,单位作业量成本明显降低;地源热泵稻谷干燥机的稻谷干燥不均度干燥前后降低幅度0.40%小于燃油稻谷干燥机的0.72%;地源热泵稻谷干燥机的破损率增加值0.16%,小于燃油稻谷干燥机的0.31%,地源热泵稻谷干燥机的爆腰率增加值2.0%,小于燃油稻谷干燥机的3.0%,干燥后的物料品质明显提高。